kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Biotinylated Mouse VEGF164 Protein, Avi Tag (Avitag™), His tag, 25µg  

Recombinant Biotinylated Mouse VEGF164 Protein, Avi Tag (Avitag™), His tag, 25µg

Recombinant Biotinylated Mouse VEGF164 Protein / VEGFA, Ala 27 - Arg 190, expressed from human 293 cells (HEK293), Avi tag (Avitag™), His tag

recombinant, mouse, biotinylated, protein, RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF

More details


Availability: within 7 days

455,00 €

Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.

Recombinant Biotinylated Mouse VEGF164, His,Avitag (VE4-M82Q3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 190 (Accession # Q00731-2).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 22.5 kDa. The protein migrates as 28-33 kDa under reducing (R) condition, and 46-60 kDa under non-reducing (NR) condition (SDS-PAGE) due to different glycosylation.

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Less than 1.0 EU per μg by the LAL method.

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Please refer to product data sheet.

Clinical and Translational Updates

(1) "Vascular endothelial growth factor isoforms are expressed in the uterus during estrous cycle of golden hamsters (Mesocricetus auratus)"
Kishi, Komatsu, Uchiyama et al
Anim Sci J (2023) 94 (1), e13804
(2) "Single dose S-ketamine rescues transcriptional dysregulation of Mtor and Nrp2 in the prefrontal cortex of FSL rats 1 hour but not 14 days post dosing"
Elfving, Liebenberg, du Jardin et al
Eur Neuropsychopharmacol (2022) 65, 56-67
(3) "VEGFR2 in vascular smooth muscle cells mediates H2S-induced dilation of the rat cerebral basilar artery"
Chen, Ding, Chen et al
Microvasc Res (2022) 141, 104309
Showing 1-3 of 278 papers.