Recombinant Biotinylated Human LILRB5 / CD85c / LIR-8 Protein, Fc Tag, Avi Tag (Avitag™), 25µg

Background
Leukocyte immunoglobulin-like receptor subfamily B member 5 (LILRB5), also known as CD85c and LIR-8, belongs to the leukocyte immunoglobulin-like receptor (LIR) family of transmembrane glycoproteins that negatively regulate immune cell activation. Several other LIR subfamily B receptors are expressed on immune cells where they bind to MHC class I molecules on antigen-presenting cells and inhibit stimulation of an immune response.

Source
Recombinant Biotinylated Human LILRB5, Fc,Avitag (CDC-H82F8) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Gly 458 (Accession # O75023-1).
Predicted N-terminus: Gly 24

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).
The protein has a calculated MW of 75.8 kDa. The protein migrates as 90-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Pharmacogenomic Study of Statin-Associated Muscle Symptoms in the ODYSSEY OUTCOMES Trial"
Murphy, Lin, Damask et al
Circ Genom Precis Med (2022) 15 (3), e003503
(2) "A Pilot Study of Whole-Blood Transcriptomic Analysis to Identify Genes Associated with Repetitive Low-Level Blast Exposure in Career Breachers"
Vorn, Edwards, Hentig et al
Biomedicines (2022) 10 (3)
(3) "Analysis of the expression and prognosis for leukocyte immunoglobulin-like receptor subfamily B in human liver cancer"
Fan, Wang, Chen et al
World J Surg Oncol (2022) 20 (1), 92
Showing 1-3 of 18 papers.