Recombinant biotinylated human LAIR2 / CD306 Protein, His Tag & Avi Tag (Avitag™), 25 µg

Background
Leukocyte-associated immunoglobulin-like receptor 2 (LAIR2) is also known as CD antigen CD306. LAIR2 contains one Ig-like C2-type (immunoglobulin-like) domain. LAIR2 is a member of the immunoglobulin superfamily and was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. LAIR2 / CD306 is thought to be secreted and may help modulate mucosal tolerance.

Source
Recombinant Biotinylated Human LAIR-2, His,Avitag (LA2-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Pro 152 (Accession # Q6ISS4-1).
Predicted N-terminus: Gln 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 17.8 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per µg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Biactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Quantitative, Spatially Defined Expression of Leukocyte-associated Immunoglobulin-like Receptor in Non-small Cell Lung Cancer"
Aung, Gavrielatou, Vathiotis et al
Cancer Res Commun (2023) 3 (3), 471-482
(2) "Association of LncRNA-PAX8-AS1 and LAIR-2 polymorphisms along with their expression with clinical and subclinical hypothyroidism"
Elsayed, Abdelazim, Darwish et al
Sci Rep (2023) 13 (1), 6
(3) "Increased concentration of soluble leukocyte-associated immunoglobulin-like receptor (LAIR) in sera from patients with blistering diseases: possible pathophysiological implications?"
Marchiano, Iervasi, Pesce et al
Minerva Med (2022)
Showing 1-3 of 39 papers.