Recombinant Biotinylated Human B7-H7 / HHLA2 Protein, Fc, Avitag™, 25 µg

Background
B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.

Source
Recombinant Biotinylated Human B7-H7, Fc,Avitag (B77-H82F5) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Asn 344 (Accession # Q9UM44-1). Predicted N-terminus: Ile 23

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).
The protein has a calculated MW of 65.9 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "Increased co-expression of stromal HHLA2 and fibroblast activation protein in upper tract urothelial carcinoma"
Nishihara, Kijima, Arai et al
Int Urol Nephrol (2023) 55 (4), 867-874
(2)  "HHLA2 promotes tumor progression by long non‑coding RNA H19 in human gallbladder cancer"
Zhang, Li, Lv et al
Int J Oncol (2022) 61 (3)
(3)  "Clinical significance and correlation of PD-L1, B7-H3, B7-H4, and TILs in pancreatic cancer"
Yang, Tian, Gao et al
BMC Cancer (2022) 22 (1), 584
Showing 1-3 of 16 papers.