Recombinant Biotinylated Human Neuropilin-1 / NRP1 / CD304 Protein, His, Avitag™, 25 µg

Background
Neuropilin-1 (NRP1) is also known as Vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells.

Source
Recombinant Biotinylated Human Neuropilin-1, His,Avitag (NR1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Phe 22 - Lys 644 (Accession # O14786-1).
Predicted N-terminus: Phe 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 73.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Evaluation of diagnostic efficacy of NRP-1/CD304 in hematological diseases"
Liu, Li, Song et al
Cancer Med (2023)
(2) "Recent advances in the tumor penetrating peptide iRGD for cancer treatment and diagnosis"
Qian, Zhou, Lin et al
Drug Dev Res (2023)
(3) "Correction: Epigenetic targeting of neuropilin-1 prevents bypass signaling in drug-resistant breast cancer"
Abdullah, Akhand, Paez et al
Oncogene (2023)
Showing 1-3 of 2253 papers.