Recombinant human CD147 Protein, His tag, 100µg

Background
CD147, also known as Basigin (BSG), or extracellular matrix metalloproteinase inducer (EMMPRIN). The human basigin protein contains 269 amino acids that form two heavily glycosylated C2 type immunoglobulin-like domains at the N-terminal extracellular portion. A second form of basigin has also been characterized that contains one additional immunoglobulin-like domain in its extracellular portion. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition and regulate several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins.

Source
Recombinant Human CD147 /EMMPRIN Protein (rhCD147), His Tag (CD7-H5222) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - His 205 (Accession # NP_940991.1).
Predicted N-terminus: Ala 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.9 kDa. The protein migrates as 26-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "The role of the C-terminal tail region as a plug to regulate XKR8 lipid scramblase"
Sakuragi, Kanai, Otani et al
J Biol Chem (2024)
(2) "Targeting cancer-derived extracellular vesicles by combining CD147 inhibition with tissue factor pathway inhibitor for the management of urothelial cancer cells"
Boddu, Zamzow, Kramer et al
Cell Commun Signal (2024) 22 (1), 129
(3) "Soluble CD147 regulates endostatin via its effects on the activities of MMP-9 and secreted proteasome 20S"
Rahat, Sabtan, Simanovich et al
Front Immunol (2024) 15, 1319939
Showing 1-3 of 2043 papers.