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Recombinant human Fas/CD95 Protein, His tag, 50µg  

Recombinant human Fas/CD95 Protein, His tag, 50µg

Recombinant Human Fas /CD95 /TNFRSF6 Protein, Gln26-Asn173, produced in human 293 cells (HEK293), His tag

Synonym
Recombinant Human Protein, FAS, ALPS1A, APO1, APT1, CD95, FAS1, FASTM, TNFRSF6, FasR

More details

FAS-H5229-050

Availability: within 7 days

336,00 €

Background
The Fas also known as FAS receptor (FasR), apoptosis antigen 1 (APO-1 or APT), cluster of differentiation 95 (CD95) or tumor necrosis factor receptor superfamily member 6 (TNFRSF6). is a death receptor on the surface of cells that leads to programmed cell death (apoptosis). It is one of two apoptosis pathways, the other being the mitochondrial pathway.[1] FasR is located on chromosome 10 in humans and 19 in mice. Similar sequences related by evolution (orthologs) are found in most mammals. Fas forms the death-inducing signaling complex (DISC) upon ligand binding. Membrane-anchored Fas ligand trimer on the surface of an adjacent cell causes trimerization of Fas receptor. This event is also mimicked by binding of an agonistic Fas antibody, though some evidence suggests that the apoptotic signal induced by the antibody is unreliable in the study of Fas signaling. To this end, several clever ways of trimerizing the antibody for in vitro research have been employed.Upon ensuing death domain (DD) aggregation, the receptor complex is internalized via the cellular endosomal machinery. This allows the adaptor molecule FADD to bind the death domain of Fas through its own death domain.[2] Recently, Fas has also been shown to promote tumor growth, since during tumor progression, it is frequently downregulated or cells are rendered apoptosis resistant. Cancer cells in general, regardless of their Fas apoptosis sensitivity, depend on constitutive activity of Fas. This is stimulated by cancer-produced Fas ligand for optimal growth.[3]

Source
Recombinant Human Fas /CD95 /TNFRSF6 Protein (rhFas) Gln26-Asn173  (Accession # AAH12479.1) was produced in human 293 cells (HEK293)

Molecular Characterization
rhFas, fused with 6×his tag at the C-terminal and has a calculated MW of 18.2 kDa expressed. The predicted N-terminal is Gln 26. Protein migrates as 25-35 kDa in reduced SDS-PAGE resulting from glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhFas by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Wajant H, 2002, Science 296 (5573): 1635–6.
(2) Huang B, et al., 1996, Nature 384 (6610): 638–41.
(3) Chen L, et al., 2010, Nature 465 (7297): 492–6.