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Recombinant human CXCR4 Protein, Fc tag, 50µg  

Recombinant human CXCR4 Protein, Fc tag, 50µg

Recombinant human CXCR4 Protein, Met1-Ser46, expressed in HEK293 cells, Fc Tag

Synonym
Recombinant human Protein, CXCR4, CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM

More details

CX4-H5269-050

Availability: within 7 days

360,00 €

Background
C-X-C chemokine receptor type 4 also known as fusin or CD184 (cluster of differentiation 184), CXCR4, CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R or WHIM. CXCR-4 is an alpha-chemokine receptor specific for stromal-derived-factor-1 (SDF-1 also called CXCL12), a molecule endowed with potent chemotactic activity for lymphocytes. This receptor is one of several chemokine receptors that HIV isolates can use to infect CD4+ T cells. HIV isolates that use CXCR4 are traditionally known as T-cell tropic isolates. Typically, these viruses are found late in infection. It is unclear as to whether the emergence of CXCR4 using HIV is a consequence or a cause of immunodeficiency.CXCR4 is upregulated during the implantation window in natural and hormone replacement therapy cycles in the endometrium, producing, in presence of a human blastocyst, a surface polarization of the CXCR4 receptors suggesting that this receptor is implicated in the adhesion phase of human implantation. SDF-1 and CXCR4 were believed to be a relatively "monogamous" ligand-receptor pair (other chemokines tend to use several different chemokine receptors in a fairly "promiscuous" manner). Recent evidence demonstrates ubiquitin is also a natural ligand of CXCR4.[1] Chronic exposure to THC increased T lymphocyte CXCR4 expression on both CD4+ and CD8+ T lymphocytes. [2] Drugs that block the CXCR4 receptor appear to be capable of "mobilizing" hematopoietic stem cells into the bloodstream as peripheral blood stem cells.


Source
Recombinant human CXCR4 Fc chimera (rhCXCR4-Fc), Met1-Ser46 (Accession # AAH20968.1) was expressed in HEK293.

Molecular Characterization
rhCXCR4-Fc is fused with Fc fragment of human IgG1 at N-terminal and has a predicted molecular mass of 32.8 kDa. In DTT-reduced SDS-PAGE, rhCXCR4-Fc protein migrates as 40-50 kDa poly peptide due to glycosylation.

Endotoxin
Less than 0.01ng endotoxin per μg rhCXCR4-Fc.

Purity
>95% purity as determined by SDS-PAGE of reduced rhCXCR4-Fc.

Formulation
Bulk protein in a 0.22 μm filtered solution of 50 mM tris, 100 mM glycine, pH7.0 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

References

(1) Saini V, Marchese A, Majetschak M, 2010, J. Biol. Chem. 285 (20): 15566–76.
(2) Lecapitaine N, et al. 2011, Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology.