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Recombinant human CD3D Protein, His tag, 100µg  

Recombinant human CD3D Protein, His tag, 100µg

Recombinant Human CD3D Protein (Phe 22-Ala 105), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant Human protein, CD3D, CD3-DELTA, T3D, T-cell-surface-glycoprotein-CD3-delta-chain

More details

CDD-H5224-100

Availability: within 7 days

378,00 €

Background
CD3D, also known as CD3-DELTA, T3D, and is a single-pass type I membrane protein. CD3D is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.[1-3] Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.[4]

Source
Recombinant Human CD3D Protein (rhCD3D) Phe 22-Ala 105 (Accession # AAH39035 ) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCD3D, fused with a C-terminal 6×his tag and has a calculated MW of 11 kDa. The predicted N-terminus is Phe 22. DTT-reduced protein migrates as 18-25 kDa polypeptide in SDS-PAGE resulting from different glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD3D by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Gil J, et al., 2011, J Clin Invest, 121(10):3872-6.
(2) Lu X, et al.,2011, Hematology, 16(3):185-9.
(3) Kim ST, et al., 2010, J Immunol, 185(5):2951-9.
(4) Holst, J. et al., 2008, Nat Immunol, 9 (6): 658-66.