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Recombinant human Fc gamma RIIIB /CD16b Protein, His tag, 100µg  

Recombinant human Fc gamma RIIIB /CD16b Protein, His tag, 100µg

Recombinant Human Fc gamma RIIIB / CD16b (NA2), His Tag (CDB-H5222) expressed from human 293 cells (HEK293)

Synonym
recombinant, human, protein Fc gamma RIIIB / CD16b (NA2), FCGR3B, CD16B, FCG3B, FCGR3, FCG3, IGFR3

More details

CDB-H5222-100

Availability: within 7 days

336,00 €

Background
CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16B also kown as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils. CD16B is the low affinity receptor for the Fc region of immunoglobulins gamma. FCGR3B binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, FCG3B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. CD16B may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Source
Human Fc gamma RIIIB / CD16b (NA2), His Tag (CDB-H5222) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Ser 200 (Accession # O75015-1).
The NA2 form of the CD16B (CDB-H5222) differ with the NA1 form of CD16B in AA36, 65, 82, and 106. The NA2 form carries S36, S65, N82, and I106, while the NA1 form (CDB-H82E4 / CDB-H5227) carries R36, N65, D82, and V106. Predicted N-terminus: Gly 17

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.7 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Immobilized Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) at 5 μg/mL (100 μl/well) can bind Biotinylated Human OX40, Fc Tag, Avi Tag (Cat. No. OX0-H82F7) with a linear range of 0.01-0.3 μg/mL (QC tested).
 

References

(1) Ravetch J.V., et al., 1989, J. Exp. Med. 170:481-497.
(2) Gessner J.E., et al., 1995, J. Biol. Chem. 270:1350-1361.
(3) Bux J., et al., 1997, Blood 89:1027-1034.