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Recombinant human CD74 / DHLAG Protein, His tag, 100µg  

Recombinant human CD74 / DHLAG Protein, His tag, 100µg

Recombinant Human CD74 Protein (Gln 73 - Met 232), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant human protein, CD74, DHLAG, HLADG, II, Ia-GAMMA, p33, CLIP, INVG34

More details

CD4-H524c-100

Availability: within 7 days

360,00 €

Background
Cluster of Differentiation 74 (CD74) is also known as HLA class II histocompatibility antigen gamma chain (HLADG), DHLAG,
Ia-GAMMA, invariant chain (Abbreviated Ii). CD74 / HLADG is a polypeptide involved in the formation and transport of MHC
class II protein. The cell surface form of the invariant chain is known as CD74. CD74 is upregulated in several cancers and is
also expressed by non-immune cells during inflammation. CD74 plays a critical role in MHC class II antigen processing by
stabilizing peptide-free class II alpha / beta heterodimers in a complex soon after their synthesis and directing transport of
the complex from the endoplasmic reticulum to the endosomal / lysosomal system where the antigen processing and binding
of antigenic peptides to MHC class II takes place. CD74 / DHLAG also serves as cell surface receptor for the cytokine MIF.

Source
Recombinant Human CD74 Protein (rhCD74) Gln 73 - Met 232 (Accession # NP_004346.1) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCD74, fused with 6×His tag at the N-terminus, has a calculated MW of 19.3 kDa. The predicted N-terminus is Gln 73. DTT-reduced Protein migrates as 23-33 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD74 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Cresswell P., 1994, Annu. Rev. Immunol. 12: 259–93.
(2) Charest A., et al., 2003, Genes Chromosomes Cancer 37:58-71.
(3) Leng L., et al., 2003, J. Exp. Med. 197:1467-1476.