Recombinant human CD74 / DHLAG Protein, His tag, 100µg

Background
Cluster of Differentiation 74 (CD74) is also known as HLA class II histocompatibility antigen gamma chain (HLADG), DHLAG,
Ia-GAMMA, invariant chain (Abbreviated Ii). CD74 / HLADG is a polypeptide involved in the formation and transport of MHC
class II protein. The cell surface form of the invariant chain is known as CD74. CD74 is upregulated in several cancers and is
also expressed by non-immune cells during inflammation. CD74 plays a critical role in MHC class II antigen processing by
stabilizing peptide-free class II alpha / beta heterodimers in a complex soon after their synthesis and directing transport of
the complex from the endoplasmic reticulum to the endosomal / lysosomal system where the antigen processing and binding
of antigenic peptides to MHC class II takes place. CD74 / DHLAG also serves as cell surface receptor for the cytokine MIF.

Source
Recombinant Human CD74, His Tag (CD4-H524c) is expressed from human 293 cells (HEK293). It contains AA Gln 73 - Met 232 (Accession # NP_004346.1).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 19.3 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD74 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Macrophage-Mediated Bone Formation in Scaffolds Modified With MSC-Derived Extracellular Matrix Is Dependent on the Migration Inhibitory Factor Signaling Pathway"
Deng, Tan, Dai et al
Front Cell Dev Biol (2021) 9, 714011
(2) "A recombinant glucocorticoid-induced leucine zipper protein ameliorates symptoms of dextran sulfate sodium-induced colitis by improving intestinal permeability"
Gentili, Hidalgo-Garcia, Vezza et al
FASEB J (2021) 35 (11), e21950
(3) "Brief report: Enhanced DRα1-mMOG-35-55 treatment of severe EAE in MIF-1-deficient male mice"
Vandenbark, Meza-Romero, Wiedrick et al
Cell Immunol (2021)
Showing 1-3 of 944 papers.