Recombinant Human CD62E / E-Selectin Protein, Fc tag, 100µg

Background
E-Selectin, also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein.[1] It is transiently expressed on vascular endothelial cells in response to IL-1β and TNFα. E selectin has a cassette structure: an N-terminal, C-type lectin domain, an EGF (epidermal-growth-factor)-like domain, 6 Sushi domain (SCR repeat) units, a transmembrane domain (TM) and an intracellular cytoplasmic tail (cyto).[2] During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. The local release of cytokines IL-1 and TNF-α by damaged cells induces the over-expression of E-selectin on endothelial cells of nearby blood vessels.[3] E-selectin mediates the adhesion of tumor cells to endothelial cells, by binding to E-selectin ligands expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, natural killer cells or cancer cells. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Source
Recombinant Human CD62E /E-Selectin Protein, Fc Tag (CDE-H5250) is expressed from human 293 cells (HEK293). It contains AA Trp 22 - Phe 556 (Accession # NP_000441.2).
Predicted N-terminus: Trp 22

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 84.8 kDa. The protein migrates as 96-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Diabetic kidney disease induces transcriptome alterations associated with angiogenesis activity in human mesenchymal stromal cells"
Bian, Conley, Eirin et al
Stem Cell Res Ther (2023) 14 (1), 49
(2) "Biomarkers of endothelial dysfunction are associated with poor outcome in COVID-19 patients: A systematic review and meta-analysis"
Mohebbi, Haybar, Nakhaei Moghaddam et al
Rev Med Virol (2023)
(3) "Endothelial Protein kinase D1 is a major regulator of post-traumatic hyperinflammation"
Schönfelder, Seibold, Morawe et al
Front Immunol (2023) 14, 1093022
Showing 1-3 of 9593 papers.