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Recombinant Human CD62E / E-Selectin Protein, Fc tag, 100µg  

Recombinant Human CD62E / E-Selectin Protein, Fc tag, 100µg

Recombinant Human CD62E / E-Selectin Protein (Trp 22 - Phe 556), produced in human 293 cells (HEK293), Fc tag

Synonym
Recombinant human protein, SELE, CD62E, ELAM1, ESEL, LECAM2, E-Selectin

More details

CDE-H5250-100

Availability: within 7 days

300,00 €

Background
E-Selectin, also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein.[1] It is transiently expressed on vascular endothelial cells in response to IL-1β and TNFα. E selectin has a cassette structure: an N-terminal, C-type lectin domain, an EGF (epidermal-growth-factor)-like domain, 6 Sushi domain (SCR repeat) units, a transmembrane domain (TM) and an intracellular cytoplasmic tail (cyto).[2] During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. The local release of cytokines IL-1 and TNF-α by damaged cells induces the over-expression of E-selectin on endothelial cells of nearby blood vessels.[3] E-selectin mediates the adhesion of tumor cells to endothelial cells, by binding to E-selectin ligands expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, natural killer cells or cancer cells. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Source
Recombinant Human CD62E /E-Selectin Protein, With C-Fc Tag (rhCD62E-Fc) Trp 22 - Phe 556 (Accession # NP_000441.2) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCD62E-Fc, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 84.8 kDa. The predicted N-terminus is Trp 22. DTT-reduced Protein migrates as 150 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD62E-Fc by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Collins T, et al., 1991, J. Biol. Chem. 266 (4): 2466–73.
(2) Graves BJ, et al., 1994, Nature 367 (6463): 532–8.
(3) Robbins SL, et al., 1999, Philadelphia: WB Saunders. ISBN 0-7216-7335-X.