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Recombinant Human CD160/BY55 Protein, 100µg  

Recombinant Human CD160/BY55 Protein, 100µg

Recombinant Human CD160 /BY55 Protein, Ile 27 - Ser 159, produced in human 293 cells (HEK293), His Tag

Synonym
CD160, BY55, NK1, NK28

More details

BY5-H5229-100

Availability: within 7 days

348,00 €

Background
CD160 antigen also known as natural killer cell receptor BY55, is a 27 kDa glycoprotein, contains 1 Ig-like V-type (immunoglobulin-like) domain. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. When expressed on vascular endothelial cells, CD160 propagates anti-angiogenic signals and promotes apoptosis.

Source
Recombinant Human CD160 /BY55 Protein (rhCD160) Ile 27 - Ser 159 (Accession # Accession # O95971-1) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.7 kDa. The protein migrates as 25-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD160 by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized Biotinylated Human HVEM, Fc Tag (Cat. No. HV4-H82F1) at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5μg/well) plate, can bind Human CD160, His Tag (Cat. No. BY5-H5229) with a linear range of 8-63 ng/mL (QC tested).

References

(1) Anumanthan A., et al., 1998, J. Immunol. 161:2780-2790.
(2) Agrawal S., et al., 1999, J. Immunol. 162:1223-1226.