Recombinant Human CD160/BY55 Protein, 100µg

Background
CD160 antigen also known as natural killer cell receptor BY55, is a 27 kDa glycoprotein, contains 1 Ig-like V-type (immunoglobulin-like) domain. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. When expressed on vascular endothelial cells, CD160 propagates anti-angiogenic signals and promotes apoptosis.

Source
Recombinant Human CD160, His Tag (BY5-H5229) is expressed from human 293 cells (HEK293). It contains AA Ile 27 - Ser 159 (Accession # O95971-1).
Predicted N-terminus: Ile 27

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.7 kDa.
The protein migrates as 25-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Biomarkers of Pathological Complete Response to Neoadjuvant Immunotherapy in Mismatch Repair Deficiency Colorectal Cancer"
Li, Hu, Qin et al
Clin Cancer Res (2023)
(2) "Expression of HPV-16 E6 and E7 oncoproteins alters Chlamydia trachomatis developmental cycle and induces increased levels of immune regulatory molecules"
Olivera, Mosmann, Anna et al
Front Cell Infect Microbiol (2023) 13, 1214017
(3) "Linking donor-specific antibody generation with natural killer cells in antibody-mediated kidney graft rejection"
Perkins, Fairchild
Kidney Int (2023) 104 (4), 644-646
Showing 1-3 of 305 papers.