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Recombinant Mouse PD-L1 /CD274 /B7-H1 Protein, His tag, 100µg  

Recombinant Mouse PD-L1 /CD274 /B7-H1 Protein, His tag, 100µg

Recombinant Mouse PD-L1 /CD274 /B7-H1 Protein, Phe 19 - Thr 238, produced in human HEK293 cells, His tag

Synonym
recombinant mouse protein PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

More details

PD1-M5220-100

Availability: within 7 days

372,00 €

Background
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

Source
Recombinant Mouse PD-L1 /CD274 /B7-H1 Protein (Mouse PDL1) Phe 19 - Thr 238 (Accession # NP_068693) was produced in human 293 cells (HEK293)

Molecular Characterization
Mouse PDL1 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 25.6 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 40-50 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse PDL1 by the LAL method.

Purity
>95% as determined by SEC-HPLC and SDS-PAGE.

Bioactivity
Immobilized Mouse PD-L1, His Tag (Cat. No. PD1-M5220) at 10 μg/mL (100 μl/well) can bind Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) with a linear range of 0.16-5 μg/mL (QC tested).

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

Bioactivity-SPR
Captured Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) on Protein A Chip can bind Mouse PD-L1, His Tag (Cat. No. PD1-M5220) with an affinity constant of 2.16 μM as determined in SPR assay (Biacore T200) (Routinely tested).
Captured Mouse PD-L1, His Tag (Cat. No. PD1-M5220) on CM5 Chip via Anti-His antibody can bind Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) with an affinity constant of 1.37 μM as determined in SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Loaded Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) on ProteinA Biosensor, can bind Mouse PD-L1, His Tag (Cat. No. PD1-M5220) with an affinity constant of 1.5 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

References

(1) Dong H., et al., 1999, Nat. Med. 5:1365-1369.
(2) Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.
(3) Nishimura H., et al., 2001, Trends in Immunology 22:265.