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Recombinant Human CD36 / SR-B3 Protein, His Tag, 100µg  

Recombinant Human CD36 / SR-B3 Protein, His Tag, 100µg

Recombinant Human CD36 / SR-B3 Protein, Gly 30 - Asn 439, produced in human 293 cells (HEK293), His tag

Synonym
recombinant, human, protein, CD36,SCARB3,GP4,GPIV,PASIV,Platelet Glycoprotein 4,glycoprotein IV

More details

CD6-H5221-100

Availability: within 7 days

312,00 €

Background
CD36 (Cluster of Differentiation 36) is also known as platelet membrane glycoprotein IV (GPIV), fatty acid translocase (FAT), thrombospondin receptor, collagen receptor, and scavenger receptor class B, member 3 (SRB3), is a member of the class B scavenger receptor family of cell surface proteins. The human CD36 gene encodes a single chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.CD36 is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils (fAβ), collagens I and IV, and Plasmodium falciparum infected erythrocytes. CD36 is required for the anti-angiogenic effects of thrombospondin1 In the corneal neovascularization assay. On binding a ligand the protein and ligand are internalized. This internalization is independent of macropinocytosis and occurs by an actin dependent mechanism requiring the activation Src-family kinases, JNK and Rho-family GTPases. CD36 ligands have also been shown to promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer.

Source
Recombinant Human CD36 / SR-B3 Protein, His Tag (CD6-H5221) is expressed from human 293 cells (HEK293). It contains AA Gly 30 - Asn 439 (Accession # NP_001001547.1).
Predicted N-terminus: Gly 30

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 48.5 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Caspase-1 Inhibition Impacts the Formation of Chondrogenic Nodules, and the Expression of Markers Related to Osteogenic Differentiation and Lipid Metabolism"
Ramesova, Vesela, Svandova et al
Int J Mol Sci (2021) 22 (17)
(2) "Hexafluoropropylene oxide dimer acid (HFPO-DA) induced developmental cardiotoxicity and hepatotoxicity in hatchling chickens: Roles of peroxisome proliferator activated receptor alpha"
Xu, Ni, Guo et al
Environ Pollut (2021) 290, 118112
(3) "High glucose‑induced upregulation of CD36 promotes inflammation stress via NF‑κB in H9c2 cells"
Han, Wang, Wu et al
Mol Med Rep (2021) 24 (5)
Showing 1-3 of 7160 papers.
Please refer to product data sheet.