Recombinant human CD156a Protein, ADAM8, His Tag, 50 µg

Background
Disintegrin and metalloproteinase domain-containing protein 8 is also known as ADAM8, Cell surface antigen MS2 and CD antigen CD156a, which belongs to the ADAM (a disintegrin and metalloprotease domain) family. ADAM family play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. ADAM8 / CD156a contains 1 disintegrin domain, 1 EGF-like domain and 1 peptidase M12B domain. ADAM8 / MS2 / CD156a is expressed on neutrophils and monocytes. ADAM8 / MS2 possible involvement in extravasation of leukocytes.

Source
Recombinant Human ADAM8, His Tag (AD8-H5223) is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Pro 497 (Accession # AAI15405.1).
Predicted N-terminus: Ile 17

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.8 kDa. Two main fragments bands are visible with apparent MW of 57-65 kDa and 35-40 kDa respectively in reduced SDS-PAGE as a result of its being prone to proteolytic cleavage.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 20 mM CaCl2, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "A Novel Class of Human ADAM8 Inhibitory Antibodies for Treatment of Triple-Negative Breast Cancer"
Mineva, Pianetti, Das et al
Pharmaceutics (2024) 16 (4)
(2) "Tnfaip8 and Tipe2 Gene Deletion Ameliorates Immediate Proteoglycan Loss and Inflammatory Responses in the Injured Mouse Intervertebral Disc"
Lu, Tian, Shofer et al
Am J Phys Med Rehabil (2024)
(3) "Changes in the Dentate Gyrus Gene Expression Profile Induced by Levetiracetam Treatment in Rats with Mesial Temporal Lobe Epilepsy"
Diaz-Villegas, Pichardo-Macías, Juárez-Méndez et al
Int J Mol Sci (2024) 25 (3)
Showing 1-3 of 301 papers.