Recombinant Human CD83 Protein, His tag, 100µg

Background
CD83 antigen is also known as B-cell activation protein and Cell surface protein HB15. CD83 is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. CD83 is expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells. CD83 may play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Source
Recombinant Human CD83, His Tag (CD3-H5223) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Ala 143 (Accession # Q01151-1).
Predicted N-terminus: Thr 20

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 14.9 kDa. The protein migrates as 17-18 kDa, 20-22 kDa and 25-30 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "The Evaluation of the Effect of Tolerogenic Probiotics on the Maturation of Healthy Dendritic Cells versus Immature Dendritic Cells"
Rastin, Mahmoudi, Tabasi et al
Iran J Immunol (2023) 20 (1), 26-35
(2) "Multi-omics analysis uncovers tumor ecosystem dynamics during neoadjuvant toripalimab plus nab-paclitaxel and S-1 for esophageal squamous cell carcinoma: a single-center, open-label, single-arm phase 2 trial"
Zhang, Yuan, Pan et al
EBioMedicine (2023) 90, 104515
(3) "Prevalence of the BCR/ABL fusion gene and T cell stimulation capacity of dendritic cells in chronic myelogenous leukemia"
Gaafar, Al-Omar, Manogaran et al
Am J Transl Res (2023) 15 (2), 967-981
Showing 1-3 of 2987 papers.