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Recombinant Human CD83 Protein, His tag, 100µg  

Recombinant Human CD83 Protein, His tag, 100µg

Recombinant Human CD83 Protein (Thr 20 - Ala 143), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant human protein, CD83,HB15,hCD83,BL11

More details

CD3-H5223-100

Availability: within 7 days

300,00 €

Background
CD83 antigen is also known as B-cell activation protein and Cell surface protein HB15. CD83 is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. CD83 is expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells. CD83 may play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Source
Recombinant Human CD83, His Tag (CD3-H5223) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Ala 143 (Accession # Q01151-1).
Predicted N-terminus: Thr 20

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 14.9 kDa. The protein migrates as 18 kDa and 22 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human CD83, His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

(1) Zhou L.-J., et al., 1992, J. Immunol. 149:735-742.
(2) Zhang Z., et al., 2004, Protein Sci. 13:2819-2824.