Recombinant Human CD98 Protein, His tag (MALS verified), 100µg

Background
CD antigen CD98 is also known as 4F2 cell-surface antigen heavy chain (4F2hc), 4F2 heavy chain antigen, Solute carrier
family 3 member 2 (SLC3A2), Lymphocyte activation antigen 4F2 large subunit, is a single-pass type I I membrane protein
which belongs to the SLC3A transporter family.. SLC3A2 / CD98 is expressed ubiquitously in all tissues tested with highest
levels detected in kidney, placenta and testis and weakest level in thymus. SLC3A2 / CD98 is required for the function of
light chain amino-acid transporters and also involved in sodium-independent, high-affinity transport of large neutral amino
acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. CD98 involved in guiding and targeting of LAT1 and
LAT2 to the plasma membrane. When associated with SLC7A5 or SLC7A8, CD98 involved in the cellular activity of small
molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane.
Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering
intracellular signals.

Source
Recombinant human CD98 Protein, His Tag (CD8-H5247) is expressed from human 293 cells (HEK293). It contains AA Arg 206 - Ala 630  (Accession # AAH01061).
Predicted N-terminus: His.

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 47.7 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Increased sodium fluorescein transport by corticosteroids is inhibited by a LAT-1 specific inhibitor in retinal pigment epithelial cells in vitro"
Misawa, Honda
Sci Rep (2023) 13 (1), 22981
(2) "Hypoxia-mimetic by CoCl2 increases SLC7A5 expression in breast cancer cells in vitro"
Canhasi, Tina, Eremo
BMC Res Notes (2023) 16 (1), 366
(3) "The role of EDIL3 in maintaining cartilage extracellular matrix and inhibiting osteoarthritis development"
Chen, Hu, Hsu et al
Bone Joint Res (2023) 12 (12), 734-746
Showing 1-3 of 1851 papers.