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Recombinant Human Ephrin-A1 / EFNA1 Protein, Fc Tag, 100 µg  

Recombinant Human Ephrin-A1 / EFNA1 Protein, Fc Tag, 100 µg

Recombinant Human Ephrin-A1 / EFNA1 Protein (Asp 19 - Ser 182), produced in human 293 cells (HEK293), Fc tag

Synonym
Recombinant human protein, EFNA1,Ephrin-A1,LERK-1,TNFAIP4,EPLG1,B61,ECKLG,EFL1

More details

EF1-H5251-100

Availability: within 7 days

300,00 €

Background
Ephrin-A1 (EFNA1) is also known as EPLG1, LERK1, TNFAIP4, Immediate early response protein B61, which belongs to the ephrin family. EFNA1 contains one ephrin RBD (ephrin receptor-binding) domain. EFNA1 is cell surface GPI-bound ligand for Eph receptors, and is a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. EFNA1 binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. EFNA1 plays an important role in angiogenesis and tumor neovascularization.

Source
Recombinant Human Ephrin-A1, Fc Tag (EF1-H5251) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Ser 182 (Accession # P20827-1).
Predicted N-terminus: Asp 19

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 46.0 kDa. As a result of glycosylation, the protein migrates as 53-57 kDa under reducing (R) condition, and 100-115 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

(1) Liu D.-P., et al., 2007, Int. J. Oncol. 30:865-871.
(2) Wykosky J., et al., 2008, Oncogene 27:7260-7273.