Recombinant Human CD163 Protein, His tag, 100 µg

Background
Scavenger receptor cysteine-rich type 1 protein M130 (CD163) is also known as hemoglobin scavenger receptor, which is a scavenger receptor for the hemoglobin-haptoglobin complex. CD163 has also been shown to mark cells of monocyte/macrophage lineage. A soluble form of the receptor exists in plasma, commonly denoted sCD163. sCD163 is generated by ectodomain shedding of the membrane bound receptor. sCD163 is upregulated in a large range of inflammatory diseases including liver cirrhosis, type 2 diabetes, macrophage activation syndrome, Gaucher's disease, sepsis, HIV infection, rheumatoid arthritis and Hodgkin Lymphoma.

Source
Recombinant Human CD163 Protein (Human CD163), His Tag (CD3-H52E8) is expressed from human 293 cells (HEK293). It contains AA Ser 42 - Ser 1045 (Accession # Q86VB7).
Predicted N-terminus: Ser 42

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 110.4 kDa. The protein migrates as 135-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Serum concentration of CD137 and tumor infiltration by M1 macrophages predict the response to sintilimab plus bevaSelection and validation of reference genes for RT-qPCR normalization of porcine alveolar macrophages (PAMs) for PRRSV studies"
Oh, De Spiegelaere, Nauwynck
Sci Rep (2023) 13 (1), 8840
(2) "Clinical, pathological, genetics and intratumoural immune milieu of micropapillary carcinoma of the colon"
Deshpande, Lee, Crabbe et al
J Clin Pathol (2023)
(3) "Spatial analysis of stromal signatures identifies invasive front carcinoma-associated fibroblasts as suppressors of anti-tumor immune response in esophageal cancer"
He, Chen, Zeng et al
J Exp Clin Cancer Res (2023) 42 (1), 136
Showing 1-3 of 5335 papers.