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Recombinant Human CD117 / c-kit Protein, His tag, 100 µg  

Recombinant Human CD117 / c-kit Protein, His tag, 100 µg

Recombinant Human CD117 / c-kit Protein, Gln 26 - Thr 516, produced in human 293 cells (HEK293), His tag

Recombinant Human protein, CD117,SCFR,c-Kit,KIT

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Availability: within 7 days

348,00 €

Mast/stem cell growth factor receptor Kit (c-Kit), a member of the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily, is also known as piebald trait protein (PBT), p145 c-kit, tyrosine-protein kinase Kit and CD117,which contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, CD117 can activate several signaling pathways. Furthermore, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B.

Recombinant Human CD117, His Tag (CD7-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Thr 516 (Accession # P10721-2).
Predicted N-terminus: Gln 26

Molecular Characterization
Human CD117, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 57.1 kDa. The predicted N-terminus is Gln 26. The reducing (R) protein migrates as 66-85 kDa in SDS-PAGE due to glycosylation.

Less than 1.0 EU per μg by the LAL method.

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Immobilized Human CD117, His Tag (Cat. No. CD7-H52H4) at 2 μg/mL (100 μL/well) can bind Biotinylated Human SCF, Avitag,His Tag (Cat. No. SCF-H82E1) with a linear range of 0.5-8 ng/mL (QC tested).


(1) Neumann I., et al., 2010, Pediatr. Blood Cancer 55:464-470.
(2) Blume-Jensen P., et al., 1994, J. Biol. Chem. 269:21793-21802.
(3) Sun J., et al.,2009, J. Biol. Chem. 284:11039-11047.