Recombinant Human CD117 / c-kit Protein, His tag, 100 µg

Background
Mast/stem cell growth factor receptor Kit (c-Kit), a member of the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily, is also known as piebald trait protein (PBT), p145 c-kit, tyrosine-protein kinase Kit and CD117,which contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, CD117 can activate several signaling pathways. Furthermore, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B.

Source
Recombinant Human CD117, His Tag (CD7-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Thr 516 (Accession # P10721-2).
Predicted N-terminus: Gln 26

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 57.1 kDa. The protein migrates as 66-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Intrathyroid thymic carcinoma: clinicopathological features and whole exome sequencing analysis"
Li, Xiang, Li et al
Virchows Arch (2023)
(2) "Unusual breast metastasis of gastrointestinal stromal tumor: A case report and literature review"
Filonenko, Karnaukhov, Kvetenadze et al
World J Clin Oncol (2023) 14 (3), 131-137
(3) "Morphological and histochemical identification of telocytes in adult yak epididymis"
Yang, Yuan, Chen et al
Sci Rep (2023) 13 (1), 5295
Showing 1-3 of 12763 papers.