Recombinant Human IFN-alpha / beta R1 Protein, Fc Tag, 100 µg

Background
Interferon alpha/beta receptor 1 ( IFNAR1) is also known as Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1 (CRF2-1), Type I interferon receptor 1, IFNAR, which belongs to the type II cytokine receptor family. IFNAR1 /IFNAR contains four fibronectin type-III domains. IFNAR1 associates with IFNAR2 to form the type I interferon receptor. IFNAR1 is receptor for interferons alpha and beta. IFNAR1 can transduce IFNB signals without the help of IFNAR2, and not activating the Jak-STAT pathway.

Source
Recombinant Human IFNAR1, Fc Tag (IF1-H5253) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # NP_000620).
Predicted N-terminus: Lys 28

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 73.8 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Downregulation of SOCS gene expression can inhibit the formation of acute and persistent BDV infections"
Li, Xia, Meng et al
Scand J Immunol (2021) 93 (1), e12974
(2) "Glucocorticoid regulation of 24-hour oscillation in interferon receptor gene expression in mouse liver"
Koyanagi, Suyama, Kuramoto et al
Endocrinology (2006) 147 (11), 5034-40
(3) "Role of interferon alpha/beta receptor chain 1 in the structure and transmembrane signaling of the interferon alpha/beta receptor complex"
Constantinescu, Croze, Wang et al
Proc Natl Acad Sci U S A (1994) 91 (20), 9602-6
Showing 1-3 of 4 papers.