Recombinant Human IFN-alpha / beta R1 Protein, Fc Tag, 100 µg

Background
Interferon alpha/beta receptor 1 ( IFNAR1) is also known as Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1 (CRF2-1), Type I interferon receptor 1, IFNAR, which belongs to the type II cytokine receptor family. IFNAR1 /IFNAR contains four fibronectin type-III domains. IFNAR1 associates with IFNAR2 to form the type I interferon receptor. IFNAR1 is receptor for interferons alpha and beta. IFNAR1 can transduce IFNB signals without the help of IFNAR2, and not activating the Jak-STAT pathway.

Source
Recombinant Human IFNAR1, Fc Tag (IF1-H5253) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # NP_000620).
Predicted N-terminus: Lys 28

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 73.8 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh IFNAR1 /IFNAR Fc Chimera by the LAL method.

Purity
90% (rh IFNAR1 /IFNAR Fc Chimera) and 13% (Human IgG1 Fc fragment) as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Colamonici O., et al., 1994, Mol. Cell. Biol. 14:8133-8142.
(2) Li X., et al., 1997, Mol. Cell. Biol. 17:2048-2056.
(3) Russell-Harde D., et al., 1999, Biochem. Biophys. Res. Commun. 255:539-544.