Recombinant Human IL-1RL1 / ST2 Protein, His tag, 200 µg

Background
Interleukin 1 receptor-like 1 (IL1RL1) is also known as ST2, DER4, FIT-1, IL33R, ST2L, ST2V, which belongs to the interleukin-1 receptor family. IL1RL1 contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RL1 is receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL1RL1 possibly involved in helper T-cell function. IL1RL1 can interact with MYD88, IRAK1, IRAK4, and TRAF6.

Source
Recombinant Human IL-1RL1, His Tag (IL1-H5229) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Phe 328 (Accession # Q01638-2).
Predicted N-terminus: Lys 19

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.9 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Osteoprotegerin and Inflammation in Incident Peritoneal Dialysis Patients"
Małecki, Okulewicz, Lisak et al
J Clin Med (2024) 13 (8)
(2) "BMP4 up-regulated by 630 nm LED irradiation is associated with the amelioration of rheumatoid arthritis"
Du, Liu, Qi et al
J Photochem Photobiol B (2024) 250, 112828
(3) "Role of IL-33-ST2 pathway in regulating inflammation: current evidence and future perspectives"
Zhou, Xu, Liu
J Transl Med (2023) 21 (1), 902
Showing 1-3 of 34 papers.