Recombinant Human NKp46 / NCR1 / CD335 Protein, Fc Tag, 50 µg

Background
Natural cytotoxicity triggering receptor 1 (NCR1) is also known as Natural killer cell p46-related protein (NK-p46), Lymphocyte antigen 94 homolog (LY94), CD antigen CD335, which belongs to the natural cytotoxicity receptor (NCR) family. NCR1 contains two Ig-like (immunoglobulin-like) domains. NCR1 interacts with CD247 and FCER1G. NCR1 / CD335 may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

Source
Recombinant Human NCR1 /NKp46 /CD335 Protein, Fc Tag (NC1-H5257) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asn 254 (Accession # AAH64806).
Predicted N-terminus: Gln 22

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh NCR1 / NKp46 / CD335 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) Siglec-7 is an indicator of natural killer cell function in acute myeloid leukemia
Yang, Feng, Wang et al
Int Immunopharmacol (2021) 99, 107965
(2) Differentiation of natural killer cells from induced pluripotent stem cells under defined, serum- and feeder-free conditions
Lupo, Moon, Chambers et al
Cytotherapy (2021)
(3) Fingolimod Therapy in Multiple Sclerosis Leads to the Enrichment of a Subpopulation of Aged NK Cells
Schwichtenberg, Wisgalla, Schroeder-Castagno et al
Neurotherapeutics (2021)

Showing 1-3 of 988 papers.
Please refer to product data sheet.