Recombinant Human NKp46 / NCR1 / CD335 Protein, Fc Tag, 50 µg

Background
Natural cytotoxicity triggering receptor 1 (NCR1) is also known as Natural killer cell p46-related protein (NK-p46), Lymphocyte
antigen 94 homolog (LY94), CD antigen CD335, which belongs to the natural cytotoxicity receptor (NCR) family. NCR1
contains two Ig-like (immunoglobulin-like) domains. NCR1 interacts with CD247 and FCER1G. NCR1 / CD335 may contribute
to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

Source
Recombinant Human NCR1 /NKp46 /CD335 Protein, with C-Fc Tag (rh NCR1 / NKp46 / CD335 Fc Chimera) Gln 22 - Asn 254 (Accession # AAH64806) was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh NCR1 / NKp46 / CD335 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Pessino A., et al., 1998, J. Exp. Med. 188:953-960.