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Recombinant Human ALCAM / CD166 Protein, Fc Tag, 200 µg  

Recombinant Human ALCAM / CD166 Protein, Fc Tag, 200 µg

Recombinant Human CD166 /ALCAM Protein (Trp 28 - Ala 526), produced in human 293 cells (HEK293), Fc tag

Synonym
Recombinant Human protein, CD166, ALCAM, CD6L, KG-CAM, MEMD

More details

CD6-H5259-200

Availability: within 7 days

324,00 €

Background
CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins, which is also known as Activated leukocyte cell adhesion molecule (ALCAM) in human, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. CD166 is a cell adhesion molecule that binds to CD6. CD166 involved in neurite extension by neurons via heterophilic and homophilic interactions.

Source
Recombinant Human CD166 /ALCAM Protein, with C-Fc Tag (rh CD166 / ALCAM Fc Chimera) Trp 28 - Ala 526 (Accession # NP_001618) was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization
rh CD166 / ALCAM Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 82.6 kDa. The predicted N-terminus is Trp 28. DTT-reduced Protein migrates as 95-120 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh CD166 / ALCAM Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Degen W.G., et al., 1998, Am. J. Pathol. 152:805-813.
(2) Skonier J.E., et al., 1996, Biochemistry 35:12287-12291.
(3) Bajorath J., et al., 1995, Protein Sci. 4:1644-1647.