Recombinant Human ALCAM / CD166 Protein, Fc Tag, 200 µg

Background
CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins, which is also known as Activated leukocyte cell adhesion molecule (ALCAM) in human, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. CD166 is a cell adhesion molecule that binds to CD6. CD166 involved in neurite extension by neurons via heterophilic and homophilic interactions.

Source
Recombinant Human CD166 / ALCAM Protein, Fc Tag (CD6-H5259) is expressed from human 293 cells (HEK293). It contains AA Trp 28 - Ala 526 (Accession # NP_001618).
Predicted N-terminus: Trp 28

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 82.6 kDa. The protein migrates as 95-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Expression of ALCAM in Clinical Colon Cancer and Relationship With Patients' Treatment Responses"
Fang, Zeng, Yang et al
In Vivo (2023) 37 (3), 1117-1128
(2) "Single-cell transcriptome analysis of NEUROG3+ cells during pancreatic endocrine differentiation with small molecules"
Li, Chen, Luo et al
Stem Cell Res Ther (2023) 14 (1), 101
(3) "Proteomic analysis of tears in dry eye disease: A prospective, double-blind multicenter study"
Jung, Kim, Song et al
Ocul Surf (2023) 29, 68-76
Showing 1-3 of 809 papers.