Recombinant Human CEACAM-5 / CD66e Protein, His Tag, 100 µg

Background
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.

Source
Recombinant Human CEACAM-5, His Tag (CE5-H5226) is expressed from human 293 cells (HEK293). It contains AA Lys 35 - Ala 685 (Accession # P06731).
Predicted N-terminus: Lys 35

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 72.2 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.1 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "Development of a highly sensitive immunoassay based on pentameric nanobodies for carcinoembryonic antigen detection"
Gu, Guo, Deng et al
Anal Chim Acta (2023) 1279, 341840
(2)  "Extracellular Vesicles Released from Cancer Cells Promote Tumorigenesis by Inducing Epithelial to Mesenchymal Transition via β-Catenin Signaling"
Malyla, Paudel, Rubis et al
Int J Mol Sci (2023) 24 (4)
(3) "Development and Characterization of an Anti-Cancer Monoclonal Antibody for Treatment of Human Carcinomas"
Tsang, Fantini, Mavroukakis et al
Cancers (Basel) (2022) 14 (13)
Showing 1-3 of 13 papers.