Recombinant Human HE4 / WFDC2 Protein, His Tag, 100 µg

Background
Human Epididymis Protein 4 (HE4) is also known as WAP four-disulfide core domain protein 2 (WFDC2), Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, is a member of the WFDC domain family. HE4 / WFDC2 is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is also a small secretory protein, which may be involved in sperm maturation.

Source
Recombinant Human HE4, His Tag (HE4-H5223) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Phe 124 (Accession # AAH46106). Predicted N-terminus: Glu 31

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 10.9 kDa. The protein migrates as 19-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1)  "HE4 overexpression in mice leads to leydig cell hyperplasia and spermatogensis impairment: Pathological implications for oligospermia"
Ling, Tang, Lin et al
Mol Cell Endocrinol (2023)
(2)  "A Dual Biomarker TK1 Protein and CA125 or HE4-Based Algorithm as a Better Diagnostic Tool than ROMA Index in Early Detection of Ovarian Cancer"
Cviič, Jagarlamudi, Meglič et al
Cancers (Basel) (2023) 15 (5)
(3)  "Performance of IOTA Simple Rules Risks, ADNEX Model, Subjective Assessment Compared to CA125 and HE4 with ROMA Algorithm in Discriminating between Benign, Borderline and Stage I Malignant Adnexal Lesions"
Czekierdowski, Stachowicz, Smolen et al
Diagnostics (Basel) (2023) 13 (5)
Showing 1-3 of 1113 papers.

Citations

(1) "Human epididymis protein 4 antigen‐autoantibody complexes complement cancer antigen 125 for detecting early‐stage ovarian cancer"
Authors: Yang WL, et al
Journal: Cancer 2019
Application: Luminex-based immunoassays