Recombinant Human CD99 / MIC2 Protein, His Tag, 100 µg

Background
CD99 antigen is also known as MIC2, single-chain type-1 glycoprotein, HBA71, MIC2X, MIC2Y, MSK5X, 12E7, which belongs to the CD99 family. CD99 / MIC2 is expressed on all leukocytes but highest on thymocytes. Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 / MIC2 plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. CD99 acts at the same site as, but independently of PECAM1. There is also experimental evidence that it binds to cyclophilin A.

Source
Recombinant Human CD99, His Tag (CD9-H5221) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 122 (Accession # AAH02584).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 10.9 kDa. The protein migrates as 25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1)  "A case of myxopapillary ependymoma with predominant giant cell morphology: A rare entity with comprehensive genomic profiling and review of literature"
Morales-Vargas, Saad, Refai et al
Neuropathology (2024)
(2)  "Resistance Improvement and Sensitivity Enhancement of Cancer Therapy by a Novel Antitumor Candidate onto A2780 CP and A2780 S Cell Lines"
Mohammadi Hadloo, Mohseni Kouchesfahani, Khanlarkhani et al
Rep Biochem Mol Biol (2023) 12 (3), 374-385
(3)  "CD99 Expression and Prognostic Impact in Glioblastoma: A Single-Center Cohort Study"
Rocca, Giudici, Donofrio et al
Cells (2024) 13 (7)
Showing 1-3 of 2227 papers.