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EGF, 500  µg  

EGF, 500 µg

Epidermal Growth Factor, human

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CK-EGF-500

Availability: within 3 days

145,00 €

The EGF ligand is expressed on cell surfaces as as transmembrane precursor. In response to physiological agonists, for example GPCR ligands and cytokines, EGF can be cleaved by membrane-anchored metalloprotease into a 53-amino acid soluble peptide. The growth factor can now bind and activate EGF receptor, leading to EGFR phosphorylation and the following activation of intracellular signaling processes including proliferation, survival, and differentiation during development, tissue homeostasis, and tumorigenesis.

Synonyms: URG, EGF

Protein informations: Epidermal Growth Factor, human recombinant produced in E.Coli, is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton. The EGF is purified by proprietary chromatographic techniques. 

Purity: > 98% by SDS PAGE

Biological activity: The ED50, calculated by the dose-dependent proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0,1 ng/ml corresponding to a specific activity of 10.000.000 Units/mg

Product specific literature references:

Higashiyama S, Iwabuki H, Morimoto C, Hieda M, Inoue H, Matsushita N. (2008) "Membrane-anchored growth factors, the epidermal growth factor family: Beyond receptor ligands." Cancer Sci. 99(2):214-20.

Schneider MR, Wolf E. (2009) "The epidermal growth factor receptor ligands at a glance." J Cell Physiol. 218(3):460-6.

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.