Recombinant human Lipocalin-2 / LCN2 Protein, His Tag, 100µg

Background
Lipocalin-2 (LCN2), also known as oncogene 24p3 or neutrophil gelatinase-associated lipocalin (NGAL), MSFI, The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores.[1-2] Lipocalin-2 also functions as a growth factor.[2] LCN2 is strongly upregulated during inflammation and is upregulated by interleukin 1 (but not TNF alpha) in humans. There are indications that some forms of acne could be caused due to the gene not being transcribed, and that Isotretinoin corrects this.[3] NFAT3 (NFATc4)NFAT by blocking the expression of LCN2 inhibits breast carcinoma cell motility[4]. Recent studies have revealed that NGAL plays an important role in the physiopathology of chronic myeloid leukaemia (CML) mediated by BCR-ABL.

Source
Recombinant human LCN2 (rhLCN2), His Tag (LC2-H5222) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Gly 198 (Accession # NP_005555.2).
Predicted N-terminus: Gln 21

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.4 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Lipocalin 2 - mutation screen and serum levels in patients with anorexia nervosa or obesity and in lean individuals"
Zheng, Rajcsanyi, Kowalczyk et al
Front Endocrinol (Lausanne) (2023) 14, 1137308
(2) "Lipocalin-2 and neutrophil activation in pancreatic cancer cachexia"
Deng, Aberle, van Bijnen et al
Front Immunol (2023) 14, 1159411
(3) "FerryCalin: an engineered lipocalin protein directed against the transferrin receptor with potential for brain drug delivery"
Nästle, Deuschle, Morath et al
Chembiochem (2023)
Showing 1-3 of 4434 papers.