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ActiveMax® Recombinant Human TGF beta1 Protein, Tag Free, 50 µg  

ActiveMax® Recombinant Human TGF beta1 Protein, Tag Free, 50 µg

ActiveMax® Recombinant Human TGF-Beta 1 / TGFB1 Protein, Ala 279 - Ser 390, expressed from human 293 cells, Tag Free

Synonyms: TGFB1, CED, DPD1 LAP, TGFB, CEDLAP, TGF beta,TGF-β1,TGF-beta1, Human Transforming Growth Factor-beta-1

More details

TG1-H4212-050

Availability: within 7 days

658,90 €

Background: Transforming growth factor beta 1 ( TGFB1) is also known as TGF-β1, CED, DPD1, TGFB. is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis.[1] The TGFB1 protein helps control the growth and division (proliferation) of cells, the process by which cells mature to carry out specific functions (differentiation), cell movement (motility), and the self-destruction of cells (apoptosis). The TGFB1 protein is found throughout the body and plays a role in development before birth, the formation of blood vessels, the regulation of muscle tissue and body fat development, wound healing, and immune system function. TGFB1 is particularly abundant in tissues that make up the skeleton, where it helps regulate bone growth, and in the intricate lattice that forms in the spaces between cells (the extracellular matrix). Within cells, this protein is turned off (inactive) until it receives a chemical signal to become active.[2-3] TGFB1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGFB1. [4] TGFB1 has been shown to interact with TGF beta receptor 1, LTBP1, YWHAE, EIF3I and Decorin.

Source
ActiveMax® Recombinant  Human TGFB1, Tag Free (TG1-H4212) is expressed from human 293 cells (HEK293). It contains AA Ala 279 - Ser 390 (Accession # P01137-1). Predicted N-terminus: Ala 279

Molecular Characterization
This protein carries no "tag". The protein has a calculated MW of 12.8 kDa (monomer). the protein migrates as 14 kDa (monomer) under reducing (R) condition, and 25 kDa (Dimer) under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg of the rhTGFB1 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN. Normally trehalose is added as protectant before lyophilization.This product does not contains polyhis tag, but there exists extremely low level of polyhis tag which might interfere with your assay when using together with anti-his tag secondary antibody.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Pemafibrate attenuates pulmonary fibrosis by inhibiting myofibroblast differentiation"
Liu, Chen, Yu et al
Int Immunopharmacol (2022) 108, 108728
(2) "Long noncoding RNA ANRIL up-regulates CCND1 via sponging miR-98-5p to promote TGF-β1-induced human airway smooth muscle cell proliferation, migration, and extracellular matrix deposition"
Zhong, Dong, Guo et al
Kaohsiung J Med Sci (2022)
(3) "[Influence of Serum TGF-β1 and EGFR Levels on the Therapeutic Effect of High-Dose AraC in Patients with Acute Myeloid Leukemia Based on the Decision Curve]"
Zhang, Liu, Huang
Zhongguo Shi Yan Xue Ye Xue Za Zhi (2022) 30 (2), 407-412
Showing 1-3 of 38407 papers.

Citations

(1) "Active traction force response to long-term cyclic stretch is dependent on cell prestress"
Authors: Cirka H, et al.
Journal: Biophys J 2016
Application: Cell Culture