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Recombinant Human Flt3-Ligand Protein, 50µg  

Recombinant Human Flt3-Ligand Protein, 50µg

Human Flt-3 Ligand Protein, AA Thr 27 - Pro 185, expressed from human 293 cells (HEK293), His Tag

Synonym: Recombinant Protein, FLT3LG, FL, FLT3L, Flt3 ligand


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Availability: within 7 days

455,00 €

FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.

Recombinant Human Flt-3 Ligand, His Tag (FLL-H5223) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # P49771-1).
Predicted N-terminus: Thr 27

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.9 kDa. The protein migrates as 28-30 kDa and 31-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Less than 1.0 EU per μg by the LAL method.

>90% as determined by SDS-PAGE. 

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Please refer to product data sheet.

Clinical and Translational Updates

(1) "Role of Flt3l and Rps15 in ketamine anesthesia"
Zhang, Xu
Medicine (Baltimore) (2024) 103 (9), e37123
(2) "FLT3L-induced virtual memory CD8 T cells engage the immune system against tumors"
Tu, Kung, Lim et al
J Biomed Sci (2024) 31 (1), 19
(3) "Identification of dendritic cell precursor from the CD11c+ cells expressing high levels of MHC class II molecules in the culture of bone marrow with FLT3 ligand"
In, Park, Shin et al
Front Immunol (2023) 14, 1179981
Showing 1-3 of 1406 papers.