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ActiveMax® Recombinant Human VEGF121, Tag Free, 50µg  

ActiveMax® Recombinant Human VEGF121, Tag Free, 50µg

ActiveMax® Recombinant Human VEGF121, Ala 27 - Arg 147, produced in human 293 cells (HEK293), Tag Free

Synonym: VEGF121, VEGFA, RP1-261G23.1, MGC70609, MVCD1, VPF, vascular endothelial growth factor (VEGF) isoform lacking heparin binding ability, VEGF protein

More details

VE1-H4213-050

Availability: within 7 days

408,00 €

Background: Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 [1, 2]. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible [4]. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis [5]. Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.

Source
ActiveMax® Human VEGF121, Tag Free (VE1-H4213) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 147 (Accession # P15692-9).
Predicted N-terminus: Ala 27

Molecular Characterization
This protein carries no "tag". The protein has a calculated MW of 14.1 kDa. As a result of glycosylation, the protein migrates as 19 kDa and 20 kDa under reducing (R) condition, and 40-45 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg of the ActiveMax® Human VEGF121 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA.
Immobilized ActiveMax® Human VEGF121 (Catalog # VE1-H4213) at 2 μg/mL can bind Human VEGF R2, Strep Tag (Catalog # KDR-H5280) with a linear range of 0.15-2.5 μg/mL.

References

(1) Byrne, A.M. et al., 2005, J. Cell. Mol. Med, 9(4):777-94.
(2) Robinson, C.J. et al., 2001, J. Cell. Sci. 114(Pt 5):853-65.
(3) Zelzer, E. et al., 2002, Development, 129(8):1893-904.