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ActiveMax® Recombinant Human TNF-alpha (HPLC-verified), 100µg  

ActiveMax® Recombinant Human TNF-alpha (HPLC-verified), 100µg

ActiveMax® Recombinant Human TNF-alpha, Val 77 - Leu 233, produced in human 293 cells (HEK293), Tag Free, low endotoxin, (active trimer), (MALS verified)

Synonyms: TNF, DIF, TNF-alpha, TNFalpha, TNFA, TNFSF2, TNF-A, cachexin, cachectin, TNF-a, TNFa, tumor-necrosis-factor-alpha, TNFa cytokine, TNF alpha protein, ADC target

More details

TNA-H4211-100

Availability: within 7 days

228,00 €

Background: 
Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages.
It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

Source
Recombinant ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (TNA-H4211) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).
Predicted N-terminus: Val 77

Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 0.1 EU per μg of the ActiveMax® Human TNF-alpha by the LAL method.

Purity
>95% as determined by SEC-HPLC.
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Dimethyl fumarate attenuates LPS induced septic acute kidney injury by suppression of NFκB p65 phosphorylation and macrophage activation"
Tang, Wang, Chen et al
Int Immunopharmacol (2021) 102, 108395
(2) "Omega 3 polyunsaturated fatty acids: Potential anti-inflammatory effect in a model of ovariectomy and temporomandibular joint arthritis induction in rats"
Marana, Benedicto Dos Santos, Groppo et al
Arch Oral Biol (2021) 134, 105340
(3) "Contribution of -1031T/C and -376G/A tumor necrosis factor alpha polymorphisms and haplotypes to preeclampsia risk in Tunisia (North Africa)"
Raguema, Ben Ali Gannoun, Zitouni et al
J Reprod Immunol (2021) 149, 103461
Showing 1-3 of 233991 papers.