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Recombinant Human Flt3-Ligand Protein,HEK293 Expressed, 50µg  

Recombinant Human Flt3-Ligand Protein,HEK293 Expressed, 50µg

Recombinant human Flt-3 Ligand / FLT3LG (rhFLT3LG) Thr27-Pro185 was produced in human HEK293 cells, ActiveMaxCytokine

Synonyms: FLT3LG, FL, FLT3L, FLT3-LG, FLT-3L, Fms-related tyrosine kinase 3 ligand, FLT3 ligand

More details

FLL-H5218-050

Availability: within 7 days

408,00 €

Background: FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand), also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1). FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts (1, 2). Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates (2). FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2-3). FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation (1, 4). Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development.[5] A lack of FLT3L results in low levels of DCs.

Source
Recombinant human Flt-3 Ligand / FLT3LG (rhFLT3LG) Thr27-Pro185 (Accession # AAI26294 ) was produced in human HEK293 cells.

Molecular Characterization
rhFLT3L, contains "NO Tag" and has a calculated MW of 18.9 kDa expressed. The predicted N-terminal is Thr27. Protein migrates as 18-30 kDa in reduced SDS-PAGE resulting from glycosylation.

Endotoxin
Less than 1.0 EU per 1 μg of the rhFLT3LG by the LAL method.

Purity
>95% as determined by SDS-PAGE. All lots are greater than 95% pure.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
The bioactivity was determined by dose-dependent stimulation of the proliferation of human acute myeloid leukemia cell line OCI-AML5.The ED50 < 0.5 ng/ml, corresponding to a specific activity of >2X106 Unit/mg.


References

(1) Wodnar-Filipowicz A., 2003, News Physiol Sci., 18:247-51.
(2) McClanahan T., et al., 1996, Blood, 88(9):3371-82.
(3) Diener K.R. et al., 2008, Exp Hematol., 36(1):51-60.
(4) Farag SS, Caligiuri MA, 2006, Blood Rev. 20(3):123-37.
(5) Shortman, Ken, et al., 2006, Nature Reviews Immunology 7 (1): 19–30.