Recombinant Mouse DKK-1 /DKK1 Protein, His tag, 50µg

Background
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts. DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression.

Source
Recombinant Mouse DKK-1 /DKK1 Protein (Mouse DKK1), His Tag (DK1-M52H6) is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 272 (Accession # O54908).
Predicted N-terminus: Thr 32

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "The plasma levels of Dickkopf-1 elevated in patients with Juvenile Idiopathic Arthritis"
Ye, Jin, Huang et al
Pediatr Res (2023)
(2) "Vascular calcification in peritoneal dialysis patients and its association with bone-derived molecules and bone histomorphometry"
Pereira, Mendonça, Magalhães et al
Nefrologia (Engl Ed) (2023)
(3) "Bone Remodeling Markers in Children with Acute Lymphoblastic Leukemia after Intensive Chemotherapy: The Screenshot of a Biochemical Signature"
Muggeo, Grassi, D'Ascanio et al
Cancers (Basel) (2023) 15 (9)
Showing 1-3 of 958 papers.