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ActiveMax® Recombinant Human GM-CSF Protein, 20µg  

ActiveMax® Recombinant Human GM-CSF Protein, 20µg

ActiveMax® Recombinant Human GM-CSF, Ala 18 - Glu 144, produced in human 293 cells (HEK293), Tag free

Synonym: Recombinant Human Protein, GM-CSF,CSF2,MGC131935

More details

GMF-H4214-20

Availability: within 7 days

192,00 €

Background
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also known as Colony stimulating factor 2 (granulocyte-macrophage), is a cytokine initially characterized by its ability to induce colonies of granulocytes and macrophages from myeloid progenitor cells, and is secreted by macrophages, T cells, mast cells, endothelial cells and fibroblasts. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Monocytes exitthe circulation and migrate into tissue, whereupon they mature into macrophages and dendritic cells. Thus, it is part of the immune/inflammatory cascade, by which activation of a small number of macrophages can rapidly lead to an increase in their numbers, a process crucial for fighting infection. The active form of the protein is found extracellularly as a homodimer. Human GM-CSF glycosylated in its mature form. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The preliminary results have been promising. GM-CSF is also used as a medication to stimulate the production of white blood cells following chemotherapy.

Source
Recombinant ActiveMax® Human GM-CSF, Tag Free (GMF-H4214) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - Glu 144 (Accession # NP_000749.2).
Predicted N-terminus: Ala 18

Molecular Characterization
This protein carries no "tag". The protein has a calculated MW of 14.5 kDa. The protein migrates as 18-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.1 EU per μg by the LAL method.

Sterility
The sterility testing was performed by membrane filtration method described in USP <71> and Eur. Ph. 2.6.1.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Imaging affective and non-affective touch processing in two-year-old children"
Maria, Hirvi, Kotilahti et al
Neuroimage (2022)
(2) "Distinct roles in phagocytosis of the early and late increases of cell surface calreticulin induced by oxaliplatin"
Matsusaka, Azuma, Kaga et al
Biochem Biophys Rep (2022) 29, 101222
(3) "Blocking the human common beta subunit of the GM-CSF, IL-5 and IL-3 receptors markedly reduces hyperinflammation in ARDS models"
Wang, Tumes, Hercus et al
Cell Death Dis (2022) 13 (2), 137
Showing 1-3 of 31212 papers.

Citations

(1) "Gut microbiota-mediated secondary bile acids regulate dendritic cells to attenuate autoimmune uveitis through TGR5 signaling"
Authors: Hu J, Wang C, Huang X, et al.
Journal: Cell reports 2021
Application: Cell culture
(2) "Linoleic acid inhibits in vitro function of human and murine dendritic cells, CD4+T cells and retinal pigment epithelial cells."
Authors: Huang X, Yi S, Hu J, et al.
Journal: Graefes Arch Clin Exp Ophthalmol 2020
Application: Cell culture
(3) "Disabled-2 (DAB2) Overexpression Inhibits Monocyte-Derived Dendritic Cells' Function in Vogt-Koyanagi-Harada Disease"
Authors: Yi, S., et al.
Journal: Immunology and Microbiology 2018
Application: Cell Culture
(4) "Decreased expression of A20 is associated with ocular Behcet's disease (BD) but not with Vogt-Koyanagi-Harada (VKH) disease"
Authors: He Y, et al.
Journal: Br J Ophthalmol 2018
Application: Cell Culture