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ActiveMax® Recombinant Human IFN-gamma protein / IFNG, 100 µg  

ActiveMax® Recombinant Human IFN-gamma protein / IFNG, 100 µg

ActiveMax® Recombinant Human IFN-gamma protein / IFNG, Gln 24 - Gln 166, produced in human 293 cells (HEK293), Tag free

Synonym: Recombinant Human Protein, Interferon-gamma, Interferon-γ

More details

IFG-H4211-100

Availability: within 7 days

180,00 €

Background
Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.

Source
ActiveMax® Recombinant Human IFN-gamma protein / IFNG, Gln 24 - Gln 166 (Accession # AAH70256) was produced in human 293 cells (HEK293)

Molecular Characterization
ActiveMax® Human IFN-gamma contains no "tag", and has a calculated MW of 16.8 kDa. The predicted N-terminus is Gln 24. The reducing (R) protein migrates as 17 kDa,20 kDa and 24 kDa in SDS-PAGE due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

Bioactivity
The bio-activity of IFN-gamma᧤Cat# IFG-H4211᧥was determined by dose-dependent inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 0.41-0.74 ng/mL.

References

(1) Gray PW, et al., 1982, Nature 298 (5877): 859–63.
(2) Miller CH, et al., 2009, Ann N Y Acad Sci.1182:69-79.
(3) Saha B, et al., 2010, Cytokine. 50(1):1-14.
(4) Murphy SP, et al., 2009, Biol Reprod. 80(5):848-59.