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ActiveMax® Recombinant Human LIF protein, Tag Free, 50 µg  

ActiveMax® Recombinant Human LIF protein, Tag Free, 50 µg

ActiveMax® Recombinant Human Leukemia inhibitory factor / LIF protein Ser 23 - Phe 202, produced in human 293 cells (HEK293), Tag Free

Synonym: Recombinant Human Protein, LIF, CDF, DIA, HILDA, MLPLI

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Availability: within 7 days

390,00 €

Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

ActiveMax® Recombinant Human LIF protein (ActiveMax® Human LIF), Tag Free(LIF-H521b) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # AAH69540.1).
Predicted N-terminus: N/A

Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 19.9 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Less than 0.1 EU per μg by the LAL method.

The sterility testing was performed by membrane filtration method.

>90% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Please refer to product data sheet.

Clinical and Translational Updates

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