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Recombinant Human Angiopoietin-like 3 / ANGPTL3 Protein, His Tag, 100 µg  

Recombinant Human Angiopoietin-like 3 / ANGPTL3 Protein, His Tag, 100 µg

Recombinant Human Angiopoietin-like 3 / ANGPTL3 / ANGPT5 Protein, Ser 17 - Pro 220, produced in human 293 cells (HEK293), His Tag

Synonym: Recombinant human protein, ANGPTL3, ANGPT5, ANG-5, Angiopoietin-5, FHBL2

More details

AN3-H5226-100

Availability: within 7 days

378,00 €

Background
Angiopoietin-like protein 3 (ANGPTL3) is also known as Angiopoietin-related protein 3, Angiopoietin-5 (ANGPT5 / ANG-5), is a member of the angiopoietin-like family of secreted factors. ANGPTL3 / ANGPT5 is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis. Angptl3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. ANGPTL3 inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels. Circulating PL-riched HDL particles have high cholesterol efflux abilities.

Source
Recombinant Human Angiopoietin-like 3, His Tag (AN3-H5226) is expressed from human 293 cells (HEK293). It contains AA Ser 17 - Pro 220 (Accession # NP_055310).
Predicted N-terminus: Ser 17

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 24.6 kDa. The protein migrates as 26-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh ANGPTL3 /ANGPT5 by the LAL method.

Purity
>95% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
Please refer to product data sheet.

References

(1) Conklin D., et al., 1999, Genomics 62:477-482.
(2) Musunuru K., et al., 2010, New England Journal of Medicine 363 (23): 2220–2227.