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Recombinant Mouse IL-10 Protein, His Tag, 20 µg  

Recombinant Mouse IL-10 Protein, His Tag, 20 µg

Recombinant Mouse IL-10 Protein, Ser 19 - Ser 178, produced in human 293 cells (HEK293), His tag

Synonym: Recombinant mouse protein, CSIF,IL-10,IL10A,TGIF,
B-TCGF,GVHDS,MGC126450,MGC126451,RP11-262N9.1,Interleukin-10

More details

IL0-M4248-20

Availability: within 7 days

192,00 €

Background
Interleukin-10 (IL-10) is also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. Mature human IL-10 shares 72% - 86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines such as IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and regulatory T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production. Knockout studies suggested the function of Interleukin-10 / IL-10 as an essential immunoregulator in the intestinal tract. Patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin-10, showing the importance of interleukin-10 for counteracting excessive immunity in the human body.

Source
Recombinant Mouse IL-10 Protein (Mouse IL-10, His Tag) Ser 19 - Ser 178 (Accession # NP_034678) was produced in human 293 cells (HEK293)

Molecular Characterization
Mouse IL-10, His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 19.6 kDa. The predicted N-terminus is His. DTT-reduced Protein migrates as 21 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse IL-10, His Tag by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

References

(1) Vieira, P. et al., 1991, Proc. Natl. Acad. Sci. 88:1172.
(2) Hsu, D.H. et al., 1990, Science 250:830.
(3) Sabat, R. et al., 2010, Cytokine Growth Factor Rev. 21:331.
(4) Blackburn, S.D. and E.J. Wherry, 2007, Trends Microbiol. 15:143.
(5) Fitzgerald DC., et al., 2007, Nat. Immunol, 8:1372.