Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human IL-29 / IFN-lambda 1 Protein, His tag, 50µg  

Recombinant Human IL-29 / IFN-lambda 1 Protein, His tag, 50µg

Recombinant Human IL-29 / IFN-lambda 1 Protein (Met 1 - Thr 200), produced in human 293 cells (HEK293), His tag

Synonyms: Recombinant human protein, IL29,IFNL1,IFNλ1,Interleukin-29,IFN lambda 1

More details

IL9-H4228-50

Availability: within 7 days

540,00 €

Background
Interleukin-29, is a secreted protein which belongs to the IL-28 / IL-29 family, and is a type III interferon. It is also known as cytokine Zcyto21, Interferon lambda-1, IFN-lambda-1, IFNλ1, IL-29 and IFNL1, and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL28A, IL28B, and IL29, also named interferonλ2 (IFNλ2), IFNλ3, and IFNλ1, respectively, are classII cytokine receptor ligands that are distantly related to members of the IL 10 family (11-13% aa sequence identity) and the type I IFN family (15-19% aa sequence identity). Human IL29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL28A and IL28B, respectively. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses and also may play a role in antiviral immunity. Interleukin-29 / IL-29 up-regulates MHC class I antigen expression. It is a Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.

Source
Recombinant Human IL-29 / IFN-lambda 1 Protein (Human IL-29, His Tag) Met 1 - Thr 200 (Accession # NP_742152.1) was produced in human 293 cells (HEK293)

Molecular Characterization
Human IL-29, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 21.1 kDa. The predicted N-terminus is Met 1 . DTT-reduced Protein migrates as 30-35 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human IL-29, His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC). 

Bioactivity
Measured in an antiviral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus.
The ED50 for this effect is typically 15 ng/mL.

References

(1) Sheppard P., et al., 2003, Nat. Immunol. 4:63-68.
(2) Kotenko S.V., et al., 2003, Nat. Immunol. 4:69-77.
(3) Vilcek J et al., 2003, Nat. Immunol. 4 (1): 8-9.