Recombinant human Sonic Hedgehog / Shh Protein, His tag, 50µg

Background
Sonic hedgehog protein (SHH) is also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

Source
Recombinant Human Sonic Hedgehog, His Tag, premium grade(SH7-H5229) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184).
Predicted N-terminus: Cys 24
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.2 EU per μg by the LAL method.

Sterility
Negative

Mycoplasma
Negative.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Cathepsin K-Positive Cell Lineage Promotes In Situ Dentin Formation Controlled by Nociceptive Sonic Hedgehog"
Xu, Zhang, Lin et al
Adv Sci (Weinh) (2024)
(2) "Neuroinflammatory Pathways Associated with Chronic Post-Thoracotomy Pain: A Review of Current Literature"
Sherbini, Hasheminia, Gemae et al
Mol Neurobiol (2024)
(3) "Primary cilia shape postnatal astrocyte development through Sonic Hedgehog signaling"
Bear, Sloan, Caspary
bioRxiv (2024)
Showing 1-3 of 7154 papers.