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Recombinant human Sonic Hedgehog / Shh Protein, His tag, 50µg  

Recombinant human Sonic Hedgehog / Shh Protein, His tag, 50µg

Recombinant Human Sonic Hedgehog / Shh Protein (Cys 24 - Gly 197), produced in human 293 cells (HEK293), His tag

Synonym
recombinant, human, SHH,HHG-1,Sonic hedgehog,HLP3,HPE3,SMMCI,TPT,TPTPS

More details

SH7-H5229-50

Availability: within 7 days

234,00 €

Background
Sonic hedgehog protein (SHH) is also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

Source
Recombinant human Sonic Hedgehog / Shh Protein (Human Sonic Hedgehog, His Tag) Cys 24 - Gly 197 (Accession # NP_000184) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
Human Sonic Hedgehog, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 20.4 kDa. The predicted N-terminus is Cys 24. DTT-reduced Protein migrates as 22 kDa in SDS-PAGE .

Endotoxin
Less than 1.0 EU per μg of the Human Sonic Hedgehog, His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Pepinsky R.B., et al., 1998, J. Biol. Chem. 273:14037-14045.
(2) Marigo V., et al., 1995, Genomics 28 (1): 44–51.
(3) Echelard Y., et al., 1993, Cell 75 (7): 1417–30.