Recombinant human Sonic Hedgehog / Shh Protein, His tag, 50µg

Background
Sonic hedgehog protein (SHH) is also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

Source
Recombinant human Sonic Hedgehog / Shh Protein (Human Sonic Hedgehog, His Tag) Cys 24 - Gly 197 (Accession # NP_000184) was produced in human 293 cells (HEK293) at ACROBiosystems. Predicted N-terminus: Cys 24

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human Sonic Hedgehog, His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

• Correia, Straub, Read et al. (2023) "Inhibition of BMP2 and BMP4 represses Barrett's esophagus while enhancing the regeneration of squamous epithelium in preclinical models" Cell Mol Gastroenterol Hepatol

• Du, Li, Yang et al. (2023) "Downregulation of Sonic hedgehog signaling induces G2-arrest in genital warts" Skin Res Technol, 29 (1), e13265

• Ebrahimi, Afshinpour, Fakhr et al. (2023) "Cancer stem cells in colorectal cancer: Signaling pathways involved in stemness and therapy resistance" Crit Rev Oncol Hematol, 182, 103920

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