Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human CX3CL1 / Fractalkine Protein, His tag, 50µg  

Recombinant Human CX3CL1 / Fractalkine Protein, His tag, 50µg

Recombinant Human CX3CL1 / Fractalkine Protein (Gln 25 - Gln 341), produced in human 293 cells (HEK293), His tag

Synonym
recombinant, human, CX3CL1, Fractalkine, cytokine, chemokine, neuractin

More details

CX1-H5221-50

Availability: within 7 days

377,00 €

Background
Fractalkine is also known as C-X3-C motif chemokine 1 (CX3CL1) and neurotactin. Fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Fractalkine is found commonly throughout the brain, particularly in neural cells, and its receptor is known to be present on microglial cells. It has also been found to be essential for microglial cell migration. CX3CL1 is also up-regulated in the hippocampus during a brief temporal window following spatial learning, the purpose of which may be to regulate glutamate-mediated neurotransmission tone. This indicates a possible role for the chemokine in the protective plasticity process of synaptic scaling.

Source
Recombinant Human CX3CL1 / Fractalkine Protein, His Tag (CX1-H5221) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Gln 341 (Accession # AAH01163).
Predicted N-terminus: Gln 25

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.5 kDa. The protein migrates as 45-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "The roles of chemokines following intracerebral hemorrhage in animal models and humans"
Wang, Bian, Du et al
Front Mol Neurosci (2022) 15, 1091498
(2) "Effect of CX3CL1/CX3CR1 gene polymorphisms on the clinical efficacy of carboplatin therapy in Han patients with ovarian cancer"
Wang, Zhou, Jiang et al
Front Genet (2022) 13, 1065213
(3) "Transient Reflexive Pain Responses and Chronic Affective Nonreflexive Pain Responses Associated with Neuroinflammation Processes in Both Spinal and Supraspinal Structures in Spinal Cord-Injured Female Mice"
Castany, Bagó-Mas, Vela et al
Int J Mol Sci (2023) 24 (2)
Showing 1-3 of 2168 papers.