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Recombinant Unconjugated Human PDGF-BB Protein, His Tag, Avi Tag (Avitag™), 50µg  

Recombinant Unconjugated Human PDGF-BB Protein, His Tag, Avi Tag (Avitag™), 50µg

Recombinant Unconjugated Human PDGF-BB Protein, Ser 82 - Thr190, expressed from E.coli cells, His Tag, Avi Tag

Synonym
recombinant, human, PDGFBB,PDGFB,FLJ12858,PDGF2,SIS,SSV,c-sis, protein

More details

PDB-H5127-50

Availability: within 7 days

360,00 €

Background
PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.

Source
Recombinant Unconjugated Human PDGF-BB, His,Avitag (PDB-H5127) is expressed from E.coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1).
Predicted N-terminus: Met

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 15.1 kDa. the protein migrates as 17 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) Hoch RV, Soriano P. 2003, Development 130 (20): 4769–4784.
(2) Olofsson B, et al. 1996, Proc Natl Acad Sci U S A.,93(6):2576-81.
(3) Joukov V, et al. 1996, EMBO J. 15 (2): 290–298.
(4) Lei KJ, et al. 1993, Oncogene 8 (4): 925–931.
(5) Ratner L, et al. 1985, Nucleic Acids Res 13 (14): 5007–18.