Recombinant Human IL-1 RAcP / IL-1 R3 Protein, His tag, 100 µg

Background
Interleukin-1 receptor accessory protein (IL1RAP or IL-1RAcP) is also known as Interleukin-1 receptor 3 (IL-1R-3 or IL-1R3), C3orf13, which belongs to the interleukin-1 receptor family. IL1RAP contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RAP is detected in liver, skin, placenta, thymus and lung. IL-1R3 / IL1RAP is coreceptor with IL1R1, associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.

Source
Recombinant Human IL-1 RAcP, His Tag (ILP-H5225) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Glu 359 (Accession # Q9NPH3).
Predicted N-terminus: Ser 21

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 40.2 kDa. The protein migrates as 50-61 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Interleukin-1 Ligands and Receptors in Lumpfish (Cyclopterus lumpus L.): Molecular Characterization, Phylogeny, Gene Expression, and Transcriptome Analyses"
Eggestøl, Lunde, Knutsen et al
Front Immunol (2020) 11, 502
(2) "Engagement of IL-1 receptor accessory protein (IL-1RAcP) with the monoclonal antibody AY19 provides co-activating signals and prolongs the CD2-induced proliferation of peripheral blood lymphocytes"
Mansur, Schiavon, Giustiniani et al
Immunol Lett (2011) 139 (1-2), 52-7
(3) "The IL1 receptor accessory protein is responsible for the recruitment of the interleukin-1 receptor associated kinase to the IL1/IL1 receptor I complex"
Volpe, Clatworthy, Kaptein et al
FEBS Lett (1997) 419 (1), 41-4
Showing 1-3 of 4 papers.