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Human IL-12B & IL-12A Heterodimer Protein, His Tag & Flag Tag, 20 µg  

Human IL-12B & IL-12A Heterodimer Protein, His Tag & Flag Tag, 20 µg

Recombinant Human IL-12B & IL-12A, His Tag & Flag Tag (IL2-H4210) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) and Arg 23 - Ser 219 (IL-12A) ((IL12B) and P29459-1 (IL12A)).

Synonym: Recombinant Human Protein, IL12, p70, Interleukin-12

More details

IL2-H4210-20

Availability: within 7 days

192,00 €

Background
Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40). IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.

Source
Human IL-12B & IL-12A, His Tag & Flag Tag (IL2-H4210) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) and Arg 23 - Ser 219 (IL-12A) (Accession # P29460-1 (IL12B) and P29459-1 (IL12A)).
Predicted N-terminus: Ile 23 (IL12B) and Arg 23 (IL12A)
Request for sequence

Molecular Characterization
The heterodimer Human IL-12B & IL-12A is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 36.6 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with polyhistidine tag at the C-terminus and subunit IL-12A is fused with flag tag at the C-terminus. The predicted N-terminus is Ile 23 (IL12B) and Arg 23 (IL12A). The reducing (R) heterodimer protein migrates as 43 kDa and 45 kDa (IL12B) and 35-40 kDa (IL12A) respectively due to glyosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Human IL-12B & IL-12A, His Tag & Flag TagHuman IL-12B & IL-12A, His Tag & Flag Tag (Cat. No. IL2-H4210) ELISA bioactivity
Immobilized Human IL-12B & IL-12A, His Tag & Flag Tag (Cat. No. IL2-H4210) at 2 μg/mL (100 μL/well) can bind Anti IL23A & IL12B P40 domain with a linear range of 0.1-2 ng/mL (QC tested).

References

(1) Kaliński P., et al., 1997, J. Immunol. 159 (1): 28–35.
(2) Hsieh CS., et al., 1993, Science 260 (5107): 547–9.
(3) Wang KS., et al., 2000, Blood 95 (10): 3183–90.