Recombinant Human CNTF R alpha Protein, His Tag, 100µg

Background
Ciliary neurotrophic factor receptor subunit alpha (CNTFR or CNTFR-alpha) belongs to the type I cytokine receptor family and type 3 subfamily. CNTFR contains two fibronectin type-III domains and one Ig-like C2-type (immunoglobulin-like) domain. This receptor and its cognate ligand support the survival of neurons. This receptor is most closely related to the interleukin-6 receptor. This receptor possesses an unusual attachment to the cell membrane through a glycophosphatidylinositol linkage.

Source
Recombinant Human CNTF R alpha, His Tag (CNR-H5225) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Pro 346 (Accession # AAH01492).
Predicted N-terminus: Gln 23

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 37.6 kDa. The protein migrates as 45-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Continuous exposure to alpha-glycosyl isoquercitrin from mid-gestation ameliorates polyinosinic-polycytidylic acid-disrupted hippocampal neurogenesis in rats"
Takashima, Okano, Ojiro et al
J Chem Neuroanat (2023) 128, 102219
(2) "Ocular manifestations of central insulin resistance"
Faiq, Sengupta, Nath et al
Neural Regen Res (2023) 18 (5), 1139-1146
(3) "Microglia-derived TNF-α mediates Müller cell activation by activating the TNFR1-NF-κB pathway"
Ji, Sun, Zhang et al
Exp Eye Res (2022) 214, 108852
Showing 1-3 of 41 papers.