Recombinant Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His, Avitag™&Flag Tag, 25 µg

Background
Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40). IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.

Source
Recombinant Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag (IL2-H8210) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) & Arg 23 - Ser 219 (IL-12A) (Accession # P29460-1(IL12B) & P29459-1(IL12A)).
Predicted N-terminus: Ile 23 (IL12B) & Arg 23 (IL12A)

Molecular Characterization
Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 38.3 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-12A is fused with flag tag at the C-terminus. The reducing (R) heterodimer protein migrates as 40-50 kDa (IL12B) and 32-35 kDa (IL12A) respectively due to glyosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Single Nucleotide Polymorphisms in Cytokine Genes are Associated with the Susceptibility to Oral Squamous Cell Carcinoma"
Pandey, Nema, Vishwakarma et al
Asian Pac J Cancer Prev (2023) 24 (7), 2353-2360
(2) "Induction of Innate Inflammatory Pathways in the Corneal Epithelium in the Desiccating Stress Dry Eye Model"
Yu, Yazdanpanah, Alam et al
Invest Ophthalmol Vis Sci (2023) 64 (4), 8
(3) "Enhanced IL-6 and IL-12B Gene Expression After SARS-CoV-2 Infection in Leprosy Patients May Increase the Risk of Neural Damage"
Santos Morais Junior, Shu Kurizky, Penha Silva Cerqueira et al
Am J Trop Med Hyg (2021) 104 (6), 2190-2194
Showing 1-3 of 58 papers.