Recombinant Canine IL-17A / CTLA-8 Protein, His Tag, 25 µg

Background
Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Source
Recombinant Canine IL-17A, His Tag (ILA-C52H7) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Ala 155 (Accession # C6L8D7-1).
Predicted N-terminus: Gly 26

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 17.0 kDa. The protein migrates as 18 kDa and 22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "Ex vivo study of IL-6 expression and function in immune cell subsets from human periapical lesions"
Džopalić, Tomić, Bekić et al
Int Endod J (2022)
(2)  "Effect of Triatoma infestans saliva on mouse immune system cells: The role of the pore-forming salivary protein trialysin"
Rocha, Gazzinelli-Guimarães, Soares et al
Insect Biochem Mol Biol (2022)
(3) "Profilin-1 prevents psoriasis pathogenesis through IκBζ regulation"
Mok, Kim, Baek et al
J Invest Dermatol (2022)
Showing 1-3 of 19564 papers.